Overview

The BI has two High Pressure Liquid Chromatography (HPLC) systems ideally suited for separation, identification, purification and quantification of complex mixtures of small molecules including proteins, peptides, pesticides, drug compounds, metabolites and, polysaccharides.

Accessories (analytical / semi-prep): Hilic columns, C18 columns, RP-Polar columns.

Waters HPLC System:
	The Waters® HPLC System includes: a 1525 Binary Pump (100 uL heads) for standard analytical flow rates to semi-preparative flow rates; an inline degasser to remove dissolved gases from liquids and primarily mobile phases; a compact and versatile Waters 2707 Autosampler module for highly precise and reproducible injections; and a Waters 2489 UV/Vis Detector which combines a high energy deuterium source,  precise optical design, low noise, and high speed electronics for dual wavelength detection from 190 to 700 nm. Control and analysis via Waters Empower software.
Vanquish HPLC System:
	The Thermo Scientific Vanquish HPLC system includes: a quaternary pump (VF-P20) with dual-pistons and vacuum degasser; a split sampler (VF-A10; capacity: 0.01 to 25uL up to 103 MPa) operating with split loop injection principles; a titanium flow cell; diode array multi-wavelength detector FG (VF-D11; range 190 nm to 800 nm with 10 signal channels); and a fluorescence detector F (VF-D50; range 200 nm to 650 nm with 4 signal channels) with single photomultiplier tube (PMT; extends range to near-infrared spectra). Control and analysis via Chromeleon software.

Note: The Vanquish is located within a CL2 lab, which requires specific biosafety training. Access to this instrument may be limited depending on lab activities.