Skip to McMaster Navigation Skip to Site Navigation Skip to main content

Microscopes – Bright Field & Fluorescence

Overview

The BI houses many light microscopes for viewing of transparent or very translucent samples. Some microscopes have fluorecence imaging capabilities.

 The Olympus upright BX53 microscope features brightfield and fluorescence light sources, fluorescence filters (DAPI, FITC, Texas Red, Cy5). Objectives include 4x, 10x, 20x, and 40x, along with two oil immersion 60x and 100x objectives*.

Images may be captured using digital QImaging Retiga 2000R cameras (monochromatic greyscale & colour) and processed using Olympus cellSens Dimension™ software.

The Olympus inverted IX51 microscope features brightfield and fluorescence light sources, fluorescence filters (DAPI, FITC, Texas Red) with 4x, 10x, 20x and 40x objectives*.

Additional filter wheel is installed for Phase Contrast microscopy.

Images may be captured using digital QImaging Retiga 2000R cameras (monochromatic greyscale & colour) and processed using Olympus cellSens Dimension™ software.

The Zeiss PrimoVert Inverted microscopes, with 4x and 10x objectives, are located within mammalian CL2 culture labs for quick and easy assessment of cells or samples.

The Zeiss AxioImager2 upright microscope features brightfield and fluorescence light sources, fluorescence filters (DAPI, FITC, Texas Red) with 4x, 10x, 20x and 40x objectives*.

Images may be captured using digital Zeiss 310 colour camera and processed using Zeiss Zen lite software.

*Certain objectives correspond to varying scales within the software.

Instrument
Uses

  • Cell imaging
  • Micro-Array Imaging
  • Histology

Sample
Requirements

  • Transparent or very translucent
  • For upright microscopes, samples are limited to being on a glass slide

System
Requirements

  • Some microscopes are within CL-2 labs which have biosafety entry requirements