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Biointerfaces Institute

Plate Reader Tecan Spark

Overview

The Tecan Spark plate reader capable of absorbance, fluorescence and luminescence measurements.

The unit can perform single wavelength measurements and/or wavelength scans, multiple reads per well, while plates are exposed to varying temperature (ambient +5°C – 42°C) and shaking (linear or orbital).

It is compatible with a variety of microplates, including 96 and 384 sizes, which may be loaded with or without the cover. Compatible with the Tecan NanoQuant plate for the quantification and fluorescent labeling efficiency of small volumes (2 ul) of nucleic acids and proteins.

Plate reader commands are programmed in Spark-control® software, while data acquisition is through Microsoft® Excel.

Instrument
Uses

  • Single Absorbance measurements and absorbance scans (230 – 1000 nm)
  • Single Fluorescence measurements, Emission scans, and Excitation scans
  • Time-Resolved Fluorescence (TRF)
  • Förster resonance energy transfer (FRET)
  • Fluorescence Polarization
  • Luminescence, single and dual channel
  • Nucleic acid and protein quantification via Nanoquant plate

Sample
Requirements

  • Sample must be contained in a micro-well plate appropriate for application

System
Requirements

  • Microplate(s) appropriate for application