Overview
Cell disruption uses high, constant pressures to compress cell samples, and accelerate them through a small fixed orifice at high velocity, in order to selectively break the cell wall and expose intracellular components. The bench-top TS-2 0.75kW model from Constant Systems is capable of pressures up to 40 kpsi resulting in approximately 10 mL stroke capacity with a 40 mL/min maximum flowrate. The disruption chamber is cooled with an external chiller (set to 4°C) to maintain sample integrity. The continuous sample run mode and electronic touch screen user interface allow for fast and easy sample disruption. Appropriate cell concentrations are recommended (eg. 25 g/L cell dry weight or 250 g/L cell wet weight).
Instrument Uses
- Cell disruption of BSL-1 material only
Requirements
- McMaster Biosafety Training – See the BI Access &Training page for more information.
- McMaster Biosafety Office approval of biological work is required
- Biointerfaces Institute internal approval of biological work is required