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Surface Characterization

The Biointerfaces Institute houses a state-of-the-art surface characterization facility featuring an Imaging and Scanning X-ray Photoelectron Spectrometer Microprobe (PHI Quantera II) which allows for large and small area spectroscopy as well as high-throughput XPS on microarrays. Additionally, the facility houses a Vertex 70 FTIR Spectrometer with a Bruker Hyperion 3000 Infrared Microscope capable of performing point imaging as well as small area imaging, an Imaging Ellipsometer (Accurion), an Imaging Surface Plasmon Resonance instrument (Horiba) which allows for high-throughput data collection and analysis, a high speed Contact Angle instrument, and a Quartz Crystal Microbalance with Dissipation (Biolin Scientific) for studying molecular interactions and surface phenomena.

We also maintain a range of Zeta Potential and Particle Sizing Instruments: a ZetaProbe (Malvern) that uses NIBS technology with Dynamic Light Scattering, a Flow Path Imaging Analyzer (Malvern) for particle size and shape analysis, and a Focused Beam Reflective Measurer (Mettler Toledo) for cord length and kinetics of flocculation.

Contact

Dr. Zeynel Bayindir

Research Lab Technician

Resource Listing

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Zeta Potential Analyzer

ZetaPALS determines zeta potential using Phase Analysis Light Scattering (PALS). PALS is used to determine the electrophoretic mobility of charged, colloidal suspensions. PALS is a technique that is 1,000 times more sensitive than traditional light scattering methods based on the shifted frequency spectrum.

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Tensile Tester

The Instron 5943 is a single column testing system built for durability and precision. The superior stiffness and alignment of this system ensures precise alignment when testing everything from biomaterials to wire or plastic film.

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Surface Plasmon Resonance Imaging

Surface Plasmon Resonance (SPR) is an established tool that offers a new generation of label-free biomolecular analysis. It can provide information on kinetic processes (association and dissociation), binding affinity, analyte concentration and real-time molecule detection.

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Quartz Crystal Microbalance with Dissipation

The Quartz Crystal Microbalance with Dissipation (QCM-D) monitoring monitors the frequency and energy dissipation response of the freely oscillating sensor. It is commonly used in surface science to characterize the formation of thin films.

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Particle Visualization Microscope

The Particle Visualization Microscope (PVM) is a probe-based vision tool that provides immediate, critical insight into crystal, particle and droplet systems. It instantly detects process changes and allows for visualization of changes to particles, droplets and cells in real-time.

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Particle Charge Detector with Automatic Titrator

The Particle Charge Detector measures colloidally dissolved substances in a process sample. Combined use with the automatic titrator allows for quantitative charge determination on colloidally dissolved matter and particle systems using a polyelectrolyte titration.

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Langmuir-Blodgett Trough

KSV NIMA Langmuir-Blodgett Deposition Troughs are the leading and most widely used instruments for Langmuir film fabrications and Langmuir-Blodgett (LB) film deposition. The LB trough can be used to create, modify, and study monolayers at either the gas-liquid or liquid-liquid interface.

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Imaging Ellipsometer

Ellipsometry is a versatile and powerful optical technique for the investigation of dielectric properties of thin films. Using an intense solid state laser (658 nm), light is reflected off of a sample and the change in the polarization of the light is detected.

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Imaging and Scanning X-Ray Photoelectron Spectrometer

Mono-energetic x-rays irradiate the sample, causing photoelectrons to be emitted from the surface. The binding energy and intensity of photoelectron peaks are detected and can provide elemental identity, chemical state information and elemental quantification.

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High Speed Contact Angle Instrument

Optical Contact Angle (OCA 35) is a contact angle measuring device that allows for automatic measurements of wetting behaviour of solids as well as a series of system analysis and tests.

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Fourier Transform Infrared Microscope

The IR beam is used to vibrationally excite molecules within the sample. The molecules are then identified based on their characteristic vibrations. The combination with microscopy allows for chemical mapping as well as chemical identification of particles and small contaminants.

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Focused Beam Reflectance Measurement

Focused Beam Reflectance Measurement (FBRM) provides the unique ability to measure particles and droplets in concentrated suspensions and emulsions, at full process concentration, without the need for sample extraction or preparation.

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Flow Particle Imaging Analyzer

The Sysmex FPIA 3000 is a fully automated dynamic flow particle imaging instrument for the rapid and reliable measurement of the shape and size of paritcles in suspension. Samples pass through the measurement cell and images of the particles are captured using stroboscopic illumination and a CCD camera.

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Dynamic Light Scattering Apparatus

The Zetasizer Nano ZS is a high performance two angle particle and molecular size analyzer for the enhanced detection of aggregates and measurement of small or dilute samples as well as samples at very low or high concentration using dynamic light scattering (DLS) with Non-Invasive Backscattering (NIBS) optics.

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Acoustophoresis Zeta Potential Analyzer

The ZetaProbe determines zeta potential the electrokinetic sonic amplitude (ESA) effect. Samples are placed in a cell containing two electrodes and the electric field (of the order 100 volt/cm) is applied at a very high frequency. The sound wave generated from the colloidal suspension is detected.